Fabrication of N,O Carboxymethyl Chltosan (NOCC)-Aldehyde Hyalnronlc Acid (AHA)-Biphasic Calcium Phosphate (BCP)-Poly (Vinyl Phosphonic Acid) (PWA) Hydrogel for Bone Regeneration

摘要

N,0 carboxymethyl chitosan-aldehyde hyaluronic acid (NOCC-AHA) hydrogel has been proved for successfully cutaneous wound healing due to its porosity, water uptake and biocompatibility. Modification of poly (vinyl phos-phonic acid) (PVPA) and biphasis calcium phosphate (BCP) nanoparticles into NOCC-AHA matrix is expected to fabricate a hydrogel for bone regeneration. BCP is main factor of osteogenesis, which shows excellent osteocon-ductivity, osteoinductivity, biodegradability. Besides, PVPA containing P-C groups in structure support bonding between bone and hydrogel matrix, and increase of bone formation by eliminating osteoclast apoptosis. This research investigates an optimal NOCC: AHA ratio loaded PVPA and BCP in order to optimize the formulation of injectable bone hydrogel. The optimal NOCC-AHA-PVPA-BCP was biocompatible, and showed the porosity, pore size were favorable for bone regeneration, which was confirmed through scanning electron microscope. However, degradation rate of this hydrogel needs improving in further research.