Photobiomodulation promotes cell survival in diabetic wounded fibroblast cells

摘要

In the presence of diabetes mellitus (DM), the wound healing process is interrupted, and chronic wounds develop. DMaffects the wound healing response and leads to early apoptosis and reduced number/function of fibroblast cells. Inthis study we investigated the hypothesis that photobiomodulation (PBM) initiates healing through reduced apoptosisand increased cell survival in diabetic wounded fibroblast (WS1) cells. Cells were irradiated using a diode laser at awavelength of 660 nm, power output of 100 mW/cm~2 and a fluence of 5 J/cm~2. Non-irradiated cells were used ascontrols. Irradiated cells were incubated for 48 h, after which assays for cell viability (Trypan blue exclusion assay),proliferation (5-Bromo-2′-deoxyuridine-BrdU), apoptosis (Annexin V/PI) and the expression of B-Cell Lymphoma 2-Bcl2 (ELISA) were done. PBM at a wavelength of 660 nm, power output of 100 mW/cm~2 and a fluence of 5 J/cm~2significantly increased cell survival through reduced apoptosis in diabetic wounded fibroblast cells. The PBM effectsobserved in this study are critical in the wound healing process.