Direct detection of cancer biomarkers using plasmonics-based Inverse Molecular Sentinel (iMS) nanobiosensors

摘要

Development of simple techniques for detection of nucleic acids is essential for clinical diagnostics. MicroRNA(miRNA) have been identified as potential biomarkers in early detection of various diseases, including esophagealadenocarcinoma (EAC) and Barrett’s esophagus (BE). However, these small molecules have not been adopted intoclinical practice because of challenging aspects in the lab. The technology described herein is a label-free nanoprobereferred to as “inverse molecular sentinel” (iMS) for gastrointestinal cancer diagnosis using surface-enhanced Ramanscattering (SERS) detection. The results of this study provide evidence supporting the robust iMS method for miRNAsensing without the need for PCR.