New Perspectives of Barcoding of Biotechnological Bacterial Strains by Using NGS Data

摘要

Next generation sequencing (NGS) technologies provided researchers with a wide variety of genetic data about organisms of interest. In this work we introduce several basic bioinformatic approaches to utilize NGS for resolving imperious problems of applied microbiology and biotechnology. Working with promising industrial strains, researches have to resolve the following questions: i) is these strain unique and if so, what makes them so unique genetically or practically speaking; ii) how can these strains be tracked down in the environment; iii) and are there any genetic markers of their extraordinary activity? All these questions may be addressed by creation genetic barcode sequences and mapping NGS reads against these barcodes.