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Sensitivity and photoperiodism response of algae-based biosensor using red and blue LED spectrums

机译:使用红色和蓝色LED光谱的基于藻类生物传感器的敏感性和光周期响应

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An algae-based biosensor is investigated using dissolved oxygen levels producing from photosynthesis mechanism and from photoperiodism response time which describes the plant's reaction to light. This paper presents the sensitivity factor in the form of dissolved oxygen rate production level and response time of green algae-based biosensor, which is stimulated using artificial light of LEDs blue (480 nm) and red (650 nm). An amperometric electrode with two green algae as bioreceptors Chlorella kessleri and Chlorella sp. were tested in the biochip C chamber. A sample of 150μL algae with a density of 5-10~5 cells/ml was illuminated by the artificial light of blue and red spectrum altered for 1000 seconds of the dark-light cycle. The results showed a significant difference in the sensitivity of the algae chlorella sp. in producing dissolved oxygen which is 6.3 and 12.9 times higher than the chlorella kessleri for the blue and red light. While the photoperiodism response of Chlorella sp. has a time constant of 49.8 % for red and 37.5 % for blue smaller than Chlorella kessleri respectively. This result shows the algae chlorella sp. possesses good response characteristics to the red light, but less for photoperiodism response.
机译:研究了使用从光合作用机制的溶解氧水平和从光学的氧气响应时间产生藻类的生物传感器,所述光周期响应时间描述植物对光的反应。本文呈现了溶解氧速率生产水平的敏感性因子和基于绿藻的生物传感器的响应时间,其使用LED蓝色(480nm)和红色(650nm)的人造光刺激。具有两个绿藻作为生物藻类小黄皮藻和小球藻的电流电极。在Biochip C室中进行了测试。密度为5-10〜5个细胞/ ml的150μl藻类的样品通过蓝色和红色的人造光照射,改变了1000秒的深光循环。结果表明藻类小球藻SP的敏感性有显着差异。在生产溶解的氧气时,该溶解氧比为蓝色和红光的小球藻kessleri高6.3和12.9倍。虽然小球藻SP的光周期反应。红色的时间常数为49.8%,蓝蓝色分别比小团kessleri小37.5%。该结果显示了藻类小球藻。对红光具有良好的反应特性,但对光周期响应的反应较少。

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