The utilization of co-solvent was considered as one of several methods to improve yield of methyl ester on in situ transesterification of microalgae.The research aims to study the effect of co-solvent in the in situ transesterification of Chlorella Sp using base catalyst under microwave irradiation.The in situ transesterification reaction carried out in a microwave reactor using 1% of base catalyst with an addition 30 ml of n-hexane as a co-solvent.The microwave was equipped with a reflux condenser and the reactor was a round bottom flask equipped with magnetic stirrer.The reaction study was performed by varying the reaction time(10,20,30,40,50)min at 450 W of microwave power.Based on the experiment result obtained that the optimal yield of methyl ester was 30.08% at 40 min.The analysis result obtained that the component of Fatty Acid Methyl Ester(FAME)from Chlorella Sp was a medium chain methyl ester(C_(12)-C_(20))with Saturated Fatty Acid(SAFA)s as the dominant component.
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