Label-free cell viability assay using lens-free microscopy

摘要

We introduce a label-free technique based on lens-free microscopy to perform cell counting and cell viability assay. Without the use of any labelling, the discrimination between alive and dead cells is obtained by analyzing the cells on the basis of their holographic signature. We have assessed this novel technique by comparing the obtained results in terms of viability and cell counting against automatic optical counting and trypan blue staining as reference methods. The lens-free measurements agree very well with the reference techniques up to ~30.10~6 cells/ml. We found a coefficient of determination R~2 of 0.99 and a slope of 1.01 for the viability measurements (N=84 CHO cell samples).