Protein Adsorption Behavior of Hydroxyapatite during Hydrothermal Synthesis

摘要

The surface structure of hydroxyapatite (HAp) particles during hydrothermal synthesis and their protein adsorption behavior were investigated. The HAp particles were prepared by mixing calcium acetate and diammonium hydrogen phosphate solutions by hydrothermal synthesis. The mixture was heated to different temperatures (120, 150, and 180 °C) and the HAp particles were collected at these temperatures. The adsorption of proteins onto the HAp particles was studied using three types of proteins: bovine serum albumin (BSA), myoglobin (MGB), and lysozyme (LSZ). Surface analysis by BET (Brunauer, Emmett, and Teller's equation) revealed that the pore volume of the HAp particles decreased with an increase in the synthesis temperature. The adsorbed amounts of BSA and LSZ (per unit milligram) were almost the same for all the HAp particles (prepared at different synthesis temperatures). In contrast, the amount of MGB adsorbed onto the HAp particles decreased with an increase in the synthesis temperature. This result suggests that the adsorbed amount of MGB decreased with a decrease in the pore volume of the HAp particles.