Translational Research of Hemoglobin-vesicles as a Transfusion Alternative

摘要

A fluid of hemoglobin-vesicles, Hb-V, is being developed as a transfusion alternative that comprises concentrated particle dispersion ([Hb] = 10 g/dL, occupied particle volume = 40%, particle size = 250 nm) with physicochemical characteristics comparable to those of blood. Encapsulation of a concentrated Hb solution with phospholipid vesicles can shield the toxic side effects of cell-free Hb, such as extravasation, NO scavenging inducing vasoconstriction and hypertension, and oxidative damage to the vascular wall. For facilitating translational research of Hb-V, we study efficient production methods, analytical methods, in vivo safety and efficacy of Hb-V (Figure 1).