Electrostransfer of Plasmid gWIZ Blank into B16-F10 and TS/A Increase Expression of Cytosolic DNA PRRs

摘要

It was observed in preclinical murine tumor models that complete tumor regression can occur when control blank plasmid DNA, not coding for a therapeutic protein, is electrotransfered into the tumor. In our study, the plasmid gWiz Blank was electrotransfered into murine melanoma (B16-F10) and mammary adenocarcinoma (TS/A) cells to determine its cytotoxic effect and expression of pattern recognition receptors (PRRs). Our in vitro results demonstrate that electrotransfer of gWiz Blank is cytotoxic for both cell lines and implicate that gene electrotransfer leads to expression or upregulation of several DNA PRRs. Additionally, increased expression of the proinflammatory cytokine IFNpi was obtained. In conclusion, activation of these sensors in tumor cells may contribute to the cytotoxicity of gene electrotransfer in vitro and inflammation and tumor regression in vivo.