Traditionally the fluorescence spectrophotometer is calibrated using chemical-made fluorescence reference materials for the ultraviolet (UV) to visible ranges and tungsten lamp for the near infrared (IR) range. Besides the inconvenience of using tungsten lamp, the common problem of many reference materials is the overlapping of the excitation and emission spectra which may induce calibration error in some wavelength rages. This article presents a LED reference source which is designed to have the same form as the sample cell usually used for the fluorescence spectrophotometer and is able to cover the whole range from UV to near IR to take the place of the inconvenient tungsten lamp and increase the measurement accuracy by avoiding the overlapping spectra problems.
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