Detection of antibodies against influenza A virus nucleoprotein (NP) in porcine oral fluid specimens using a commercial blocking ELISA

摘要

In contemporary commercial swine populations, influenza is increasingly recognized as an endemic virus, an important component of the porcine respiratory disease complex (PRDC), and a pathogen with a major economic impact on swine production. Previously, a commercial blocking ELISA (MultiS-Screen~R Antibody Test Kit, IDEXX Laboratories, Inc.) designed to detect anti-influenza A nucleoprotein (NP) antibodies in avian serum was shown to effectively detect anti-NP antibodies in swine serum. The purposeof present study was to determine whether the "NP ELISA" could also be adapted to the detection of anti-NP antibodies in swine oral fluid samples.