Effect of Trace Element Copper Supplemented in Medium on Growth, Yield, and Physiology of Pleurotus eryngii

摘要

Trace element,copper,was added into the culture medium of Pleurotus eryngii at different concentrations for revealing effects of Cu on growth and physiology of P. eryngii. The growth rate of mycelium, quality of fruit body,and activity of some enzymes of P. eryngii were detected in various development periods. The fluxes of Ca2+, H+, and K+on the surface of mycelium cell were determined by NMT technology. The results show that the mycelium growth rate did not change under addition of Cu at the concentration of 20-100mg/kg,and the fruit body yield of P. eryngii was raised at a certain concentration range. When the concentration of Cu was 60mg/kg,the unit yield (169.18 g) of P. eryngii was the highest and it was 16.45% higher than that of the control group. At the concentration of 20-100mg/kg, Cu can promote the activities of nitrate reductase (NR), glutamate-pyruvate transaminase (GPT) and sucrose phosphate synthase (SPS) in P. eryngii. The extracellular H+flux was accelerated by adding Cu,the maximum flux reached to 0.587 pmol/(cm ? s) from budding period to harvesting period. The extracellular K+flux rate was accelerated at certain stage,but Cu at high concentrations inhibited the flux speed of K+. By adding Cu in medium, Ca2+changed from release state to absorption state at budding period, and was at absorption state during harvesting period.