A novel method for viability enumeration for single-droplet drying of Lactobacillus plantarum WCFS1

摘要

An experimental approach to assess the microbial viability within single particles is presented.It comprises a single droplet drying method and a subsequent novel live-dead screening methodology.The drying method involves the drying of a single droplet deposited on a flat,hydrophobic surface under well-defined drying conditions and carrier formulations.(Semi-)dried particles were subjected to rehydration,fluorescence staining,and live/dead enumeration using fluorescence microscopy.The novel screening methodology provided accurate survival percentages in line with conventional plating enumeration and was evaluated in single droplet drying experiments with Lactobacillus plantarum WCFS1 as a model probiotic strain.