MICROFLUDIC CHIP WITH THREE-DIMENSIONAL HYDRODYNAMIC FOCUSING FOR HIGH-THROUGHPUT SINGLE-CELL ANALYSIS WITH CONTINUOUS CELL INTRODUCTION AND RAPID DYNAMIC LYSIS

摘要

A cross microchip with three sheath-flow channels located on both sides of and below the sampling channel was developed for high-throughput single-cell analysis. The sample stream containing labeled carcinoma cells is constrained by the underneath sheath-flow stream to an upper layer, thus avoiding channel blockage by adhered cells. With electric field applied on the separation channel, the aligned cells were continuously driven into the separation channel and rapidly lysed within 400 ms by sodium dodecylsulfate. Subsequently, L-Glutathione and reactive oxygen species in single cells were electrophoretic separated and detected by LIF. The maximum rate of 151 cells min~1 was achieved.