A NOVEL FULLY-AUTOMATED MICROFILTER PLATFORM USING SELECTIVE SIZE AMPLIFICATION OF CIRCULATING TUMOR CELLS

摘要

From continuous attention to conquer metastatic cancers, the detection of circulating tumor cells (CTCs) is believed to provide potent clues for prediction and prognosis of cancers and tailored therapy. This paper introduces a novel CTC isolation method using selective size amplification for target cells and multi-obstacle structure (MOS) filters. Microbeads conjugated with anti-EpCAM amplified the size of MCF-7 breast cancer cells over 6 μm in diameters and the clearly discriminated cancer cells with white blood cells (WBCs) in size were stably captured from the filter. The MOS platform satisfying stability and high recovery rate is expected to contribute sensitive and credible clinical validations of CTC studies.