MICRO-OPTO-FLUIDIC-SYSTEM (MOFS) FOR LABEL-FREE DETECTION OF WATERBORNE PATHOGENS IN DRINKING WATER

摘要

This paper reports a micro-opto-fluidic-system (MOFS) for real-time, high throughput and label-free detection of single particles in drinking water. The proposed system is based on a high flow rate achievable microfluidic chip and on chip multiple angle scattering (MALS) detection, which can detect the size and refractive index of single particle at flow rate as high as 10L/h. In the demonstrated experiment, polystyrene microspheres with different sizes under different solutions are detected and the size and RI measurements have precision of 0.1 μm and 0.005 respectively. Besides, real waterborne pathogens and particles such as Cryptosporidium parvum occysts, Giardia lamblia cysts, Escherichia Coli, sands and silts in drinking water are also detected by the system. The scattering patterns of different samples are distinctive and these results suggest that the waterborne pathogens can be easily detected and identified by the MOFS.