CELL LYSIS BY LOW POWER FOCUSED ACOUSTIC TRANSDUCER AND INVESTIGATION OF ACOUSTIC INTENSITY THRESHOLD FOR CYTOLYSIS OF VARIOUS CELL LINES

摘要

This paper reports recent development of micron-sized cell lysis without noticeable acoustic cavitation by a Self Focused Acoustic Transducer (SFAT). Localized cell lysis was obtained with prostate cancer cell lines and breast cancer cell lines by applying various power intensity levels at the transducer's fundamental and 3rd harmonic resonant frequencies (17.3 and 56 MHz, respectively). The cell lysis area was around 100 μm (fundamental) and 50 μm (third harmonic) in diameter. According to the results, the cell lysis area depended on the acoustic-wave frequency, but not on the applied acoustic intensity above the threshold intensity. Also, different cell lines showed varied sensitivity to focused acoustic energy, and had different energy threshold for localized cytolysis.