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The Effects of Low-level Arsenic Exposure on Erythrocyte Membrane Proteins in Male Rats

机译:低水平砷暴露对雄性大鼠红细胞膜蛋白的影响

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The rats were exposed to arsenic in drinking water (10, 60, 360μg/L) for 30 days. As shown in SDS-PAGE, compared with the control group, ankyrin content was significantly decreased in 360μg/L dose group. With exposure levels rising, band 3 protein expression presented an increasing trend in the treated groups. Immunofluorescence analysis revealed that, in 60μg/L and 360μg/L arsenic treatment groups, band 3 and CD3S increased and clustered to the RBC membrane. The results for rat erythrocyte parameters revealed that the abnormal changes have not occurred in the exposed groups. This study suggests that the injuries in RBC membrane proteins occur earlier than the abnormal changes in RBC number and volume. Erythrocyte membrane proteins may be the early biomarkers of chronic arsenic poisoning.
机译:将大鼠暴露于饮用水中的砷(10,60,360μg/ L)30天。如SDS-PAGE所示,与对照组相比,360μg/ L剂量组中的Ankyrin含量显着降低。随着曝光水平上升,带3蛋白表达呈现治疗组中的趋势。免疫荧光分析显示,在60μg/ L和360μg/ L砷处理基团中,带3和CD3S增加并聚集到RBC膜中。大鼠红细胞参数的结果表明,暴露基团中未发生异常变化。该研究表明,RBC膜蛋白的损伤比RBC数量和体积的异常变化发生。红细胞膜蛋白可以是慢性砷中毒的早期生物标志物。

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