(cest2017_00358) Study of bacterial population dynamics in sequence batch reactors under different operating conditions in the presence of a metabolic uncoupler, 3,3',4',5-tetrachlorosalicylanilide (TCS)

摘要

The process of uncoupled metabolism has been studied since the ?90s for the reduction of sludge production. Under normal growth conditions, the catabolism and anabolism of bacteria are coupled, however under uncoupled conditions (brought on by chemical uncouplers), the catabolism is undisturbed leading to unchanged substrate consumption, while the ATP synthesis and anabolism slow down causing reduction in biomass yield. The 3,3',4',5-tetrachlorosalicylanilide (TCS) has been widely adopted as an environmentally-benign uncoupler to reduce yield of activated sludge. However, its potential impact, in the microbial community of SBR activated sludge, is unknown yet. Four parallel sequence batch reactors (SBR) with and without TCS addition were operated under different conditions, two F/M ratios, to research the microbial population dynamics by uncouplers. The 16S rRNA gene amplicon sequencing (NGS) was performed to reveal the microbial community. During the 41 days operation, the TCS of the SBR was 1 mg/L. Specific comparisons down to the family, class and genus level were done from Illumina-MiSeq amplicons. This study provides a general view of the composition of microbial communities in activated sludge of SBR operated under different conditions with TCS