Nanoparticles of Molybdenum Chlorophyllin Photosensitizer and Magnetic Citrate-Coated Cobalt Ferrite Complex Available to Hyperthermia and Photodynamic Therapy Clinical Trials

摘要

This study report on the synthesis and characterization of molybdenum chlorophyllin (Mo-Chl) compounds associated in a complex with magnetic nanoparticles (citrate-coated cobalt ferrite), the latter prepared as a biocompatible magnetic fluid (MF). The complex material was developed for application as a synergic drug for cancer treatment using Photodynamic Therapy (PDT) and Hyperthermia (HPT). Chlorophyllin was obtained from alkaline extraction of Ilex paraguariensis following molybdenum insertion from hydrolysis with molybdate sodium. Fluorescence quantum yield (Φ_f) of Mo-Chl/dimethyl-sulphoxide (DMSO) was lower than 0.1, with a lifetime of 5.0 ns, as obtained from time-correlated single-photon counting technique. The oxygen quantum yield of Mo-ChI was carried out using laser flash-photolysis studies in homogeneous medium saturated with O_(2(g)) (Φ_Δ = 0.50). Cellular viability was also evaluated via the classical MTT assay using gingival fibroblasts cells as a biological model. Studies performed with the complex Mo-Ch1(5.0 μmol.L~(-1))/MF at different magnetic nanoparticle concentrations (ranging from 10~(12) to 10~(15) particle.mL~(-1)) revealed a cellular viability of approximately 95% for the ideal magnetic material concentration of 1 x 10~(13) particle.mL~(-1). The present study shows that natural photosensitizers molecules Mo-ChI used in association with magnetic nanoparticles represent a promising generation of drug developed to work synergistically in the treatment of neoplastic tissues using PDT and HPT.