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Elegant Synthesis Strategies Using a New Magnetic Bed Reactor: Monoclonal Mouse anti-CD11b Derivatized Superparamagnetic Iron Oxide Nanoparticles

机译:优雅的合成策略采用新型磁床反应器:单克隆鼠标抗CD11b衍生化超顺磁性氧化铁纳米粒子

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The new magnetic reactor based solid phase synthesis strategy allows magnetic immobilization of nanoparticles in order to perform the derivatization step(s) with relevant biomolecules on the immobilized magnetic nanoparticles. Monoclonal Mouse anti-Human CD I lb shows affinity toward monocyte/macrophage integrin MAC-1 (CD11b/CD18) which is involved in leukocyte adhesion and functions as a cell receptor and was coupled on superparamagnetic iron oxide nanoparticles (SPIONs) in this reactor. The resulting anti-CD 1 lb derivatized SPIONs were analyzed and compared to conventionally derivatized SPIONs. The reactor-derivatized anti-CD11b-SPIONs show enhanced colloidal stability during and after the surface derivatization steps. The yield and quality of the resulting particles can also be significantly improved in terms of reaction time and yield, particle size distribution, and scalability by using this magnetic reactor. These highly specific particles were successfully used in imaging studies of monocytes.
机译:新的反应器磁基于固相合成策略允许纳米颗粒的磁性固定,以便执行与在固定化磁性纳米颗粒相关的生物分子衍生化步骤(一个或多个)。单克隆小鼠抗人CD I LB显示朝向单核细胞/巨噬细胞整合蛋白MAC-1(CD11B / CD18)的亲和力,其参与白细胞粘附和用作细胞受体,并在该反应器中偶联在超顺磁性氧化铁纳米颗粒(胶片)上。分析并将得到的抗CD11B衍生栓分析并与常规衍生酱进行比较。反应器 - 衍生化的抗CD11B-悬油屏在表面衍生步骤期间和之后显示出增强的胶体稳定性。在反应时间和产率,粒度分布和通过使用该磁反应器,也可以显着改善所得颗粒的产率和质量。这些高度特异性颗粒已成功用于单核细胞的成像研究。

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