Elegant Synthesis Strategies Using a New Magnetic Bed Reactor: Monoclonal Mouse anti-CD11b Derivatized Superparamagnetic Iron Oxide Nanoparticles

摘要

The new magnetic reactor based solid phase synthesis strategy allows magnetic immobilization of nanoparticles in order to perform the derivatization step(s) with relevant biomolecules on the immobilized magnetic nanoparticles. Monoclonal Mouse anti-Human CD I lb shows affinity toward monocyte/macrophage integrin MAC-1 (CD11b/CD18) which is involved in leukocyte adhesion and functions as a cell receptor and was coupled on superparamagnetic iron oxide nanoparticles (SPIONs) in this reactor. The resulting anti-CD 1 lb derivatized SPIONs were analyzed and compared to conventionally derivatized SPIONs. The reactor-derivatized anti-CD11b-SPIONs show enhanced colloidal stability during and after the surface derivatization steps. The yield and quality of the resulting particles can also be significantly improved in terms of reaction time and yield, particle size distribution, and scalability by using this magnetic reactor. These highly specific particles were successfully used in imaging studies of monocytes.