Quantification of 1,3-Butadiene-Induced DNA adducts in Human Blood Using Liquid Chromatography-High Resolution Tandem Mass Spectrometry (HPLC-HR MS/MS)

摘要

Highly sensitive capillary HPLC-ESI~+-HRMS/MS-SRM methods for and nanoHPLC-NSI~+-HR-MS~3 methods for EB-Gua in human DNA have been developed and validated. No significant differences were observed between N7-THBG adduct levels in leukocyte DNA of smokers and nonsmokers (P = 0.60,). Furthermore, smoking cessation did not alter N7-THBG concentrations in human leukocyte DNA, suggesting that BD exposure sources other than smoking contribute to N7-THBG formation in humans. In contrast, N7-THBG adduct concentrations were significantly increased in workers occupationally exposed to BD as compared to controls. Sensitive methodology developed in this work will be used for future studies of BD adduct formation in humans, facilitating human risk assessment from exposure to BD.