Proteomic Analysis of Perineural Invasion in Pancreatic Adenocarcinoma Reveals Up-Regulation of Neurosecretory Protein VGF in Invaded Nerves

摘要

Combination of laser microdissection and mass spectrometry successfully identified thousands of proteins from the microscopic lesions of PNI. Whilst large number of proteins were differentially regulated in invaded compared to non-invaded nerves, the comparison of PNI and non-PNI cancer revealed only small number of deregulated proteins. The up-regulation of Neurosecretory protein VGF in IN was validated using IHC. Parallel 1D and 2D/LC analytical approaches allow for greater confidence in deciding candidate biomarkers. Parallel data processing using both NSAF and LFQ intensities allows for the advantages of both approaches to be exploited.