Affinity Mesh Screen Materials for Rapid Drug Discovery using Transmission Mode Desorption Electrospray Ionization (TM-DESI) Mass Spectrometry

摘要

The fluorine peak observed in the XPS data verifies the successful coating of the polyacrylate film onto the mesh screen by PPP, and its subsequent functionalization. Vancomycin was retained even after the washing step was incorporated into the preparation scheme, while spectinomycin, which does not bind to Kaa, was less retained. The solution desorption plots show noticeable difference between Kaa binding antiobiotics and non-Kaa binding antibiotics indicating that the functionalized screens are capable of selective binding. A series of different diastereomer functionalized screens (KAa; KaA) were synthesized as well as mutant variants of the Kaa tripeptide sequence (K-a-s), which can be rapidly screened with this developed method to probe binding interactions between different antibiotics. This work can be expanded even further to analysis of a simulated "mock" natural extract solution in order to demonstrate its applicability to select potential new antibiotic agents from complex matrices in solution.