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Targeted Metabolomics - a Simple and Fast Method for the Analysis of Water and Fat Soluble Vitamins in Cell Culture Media Using HPLC-ESI-MSMS

机译:有针对性的代谢组学 - 一种使用HPLC-ESI-MSMS分析细胞培养基中水和脂溶性维生素的简单且快速的方法

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摘要

A simple method for the exact quantification of 12 water soluble and 4 fat soluble vitamins/vitaminoids in cell culture media samples has been developed. With a total runtime of 20 min per sample and very fast and simple sample preparation procedure, a high-throughput analysis can be easily achieved. The method is being validated and has been successfully applied in many metabolomic studies.
机译:已经开发出一种简单的12水溶性和4种脂溶性维生素/维生素在细胞培养介质样品中的精确定量方法。 每种样品20分钟的总运行时间和非常快速简单的样品制备程序,可以容易地实现高通量分析。 该方法正在验证,并已成功应用于许多代谢组研究。

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