The use of hydrazine based derivatisation reagents for improved sensitivity and detection of carbonyl containing compounds 'on tissue' using MALDI-MS

摘要

The microscope images in Figure 1 show the crystal formation of the 2,4-Dinitrophenylhydrazine reactive matrix observed under the current conditions described. These images show that under these conditions the crystals are not uniform or evenly distributed, as would be expected for those of more conventional MALDI matrices. Figure 2 shows the MALDI-MS images of the distribution of the radical cation ([M]~(+centre dot)) m/z 500, hydrazone derivative ([M_(D)+H]~(+)) m/z 681, sodium adduct ([M+Na]~(+)) m/z 523 and potassium adduct ([M+K]~(+)) m/z 539 of Fluticasone Propionate within the spiked areas of the lung tissue. These images show that the reactive matrix can be used to produce MALDI-MS imaging data. However, the lack of linearity of the response with respect to concentration spiked needs to be investigated further. The cause may be related to the compound spiking methods and / or matrix application.