Lipidomic Profiling of Rat Adipose Tissue after Treatment with PPAR-pan Agonist using Sub-2μm Particle CO_2 Based Supercritical Chromatography

摘要

The organics phase lipid extract can be directly injected into the UPC~2 system saving sample prep time and reducing cost per analysis. Significant saving in chromatographic separation time for both FFA and TAG (10X faster compared to GC/MS). No derivitization required which results in easier and fast sample prep and eliminated of artifact formation. UPC~2 provides an alternative method for lipid class separation. UPC~2 provide a single technique for the separation of polar and non-polar lipids with a simple switch of the column and gradient, thus enabling to combine two or more techniques into one. The developed method can be applied for lipid profiling in pharmaceutical, food and chemical samples.