Utilizing mass spectrometry-based profiling system to identify cellular response proteins induced by silk fibroin surface-modified biomaterials

摘要

The purpose of this study was to develop the formation of silk fibroin (SF) biopolymer surface, and the fibroblasts were used as an experimental model to evaluate the responses of cellular proteins induced by biopolymer material using a mass spectrometry-based profiling system. The surface was covered by multi-walled carbon nanotubes (CNT) and SF to increase the surface area, enhance the adhesion of biopolymer and promote the rate of cell proliferation. The amount of adhered fibroblasts on CNT/SF electrodes of quartz crystal microbalance (QCM) were greatly exceeded those on other surfaces. Moreover, analyzing differential protein expressions of adhered fibroblasts on the biopolymer surface by proteomic approaches was indicated that CD44 may be a key protein. Through this study, utilizing mass spectrometry-based proteomics in evaluation of cell adhesion on biopolymer was proposed.