Improved proteome coverage using novel data-independent acquisition strategies in ion mobility LC-MS experiments

机译：使用新型数据无关的采集策略改进了离子移动性LC-MS实验的蛋白质组覆盖率

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Ion mobility-dependent CE profiles in nanoUPLC-IMS-MS (UDMSE) facilitate deep proteome coverage in DIA proteomics Increase of identifiable proteins by >40% compared to classical HDMSE Reproducible iden5fication of >4400 proteins in 180 min gradient time in triplicate injections (searchiing UniProtKB/Swissprot) Similar if not exceeding levels of performance compared to DDA-based workflows on Orbitrap instruments regarding sensitivity, number of identified proteins and peptide identification rates.

机译：与180分钟的梯度时间中的典型HDMSE可再现的IDEN5校正在三份注射中的典型HDMSE可再现IDEN5，促进纳米蛋白质组学（UDMSE）中的离子络合蛋白质组（UDMSE）中的深度蛋白质组学覆盖促进蛋白质组学覆盖。 Uniprotkb / swissprot）与关于含有关于敏感性的基于DDA仪器上的基于DDA的工作流程相比，类似的，鉴定的蛋白质和肽识别率的数量相比，类似的情况相似。

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《American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics》|2013年||共1页
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Ute Distler; Jorg Kuharev; Stefan Tenzer;
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中图分类 TB994.5-53;
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1. Data-independent acquisition (MSe) with ion mobility provides a systematic method for analysis of a bacteriophage structural proteome. [J] . Moran D., Cross T., Brown L. M., Journal of Virological Methods . 2014,第Null期

机译：具有离子迁移率的数据独立采集（MS e ）为分析噬菌体结构蛋白质组提供了一种系统的方法。
2. Ranking Fragment Ions Based on Outlier Detection for Improved Label-Free Quantification in Data-Independent Acquisition LC-MS/MS [J] . Bilbao Aivett, Zhang Ying, Varesio Emmanuel, Journal of proteome research . 2015,第11期

机译：基于离群值检测对碎片离子进行排序，以改进独立于数据的采集LC-MS / MS中的无标记定量
3. MdFDIA: A Mass Defect Based Four-Plex Data-Independent Acquisition Strategy for Proteome Quantification [J] . Yi Di, Ying Zhang, Lei Zhang, Analytical chemistry . 2017,第19期

机译：MDFDIA：蛋白质组量化的基于大规模缺陷的四个Plex数据无关的采集策略
4. Improved proteome coverage using novel data-independent acquisition strategies in ion mobility LC-MS experiments [C] . Ute Distler, Jorg Kuharev, Stefan Tenzer American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2013

机译：使用新型数据无关的采集策略改进了离子移动性LC-MS实验的蛋白质组覆盖率
5. Mining deeper into the proteome: Computational strategies for improving depth and breadth of coverage in high-throughput protein identification studies. [D] . Ulintz, Peter J. 2008

机译：深入蛋白质组学：在高通量蛋白质鉴定研究中提高覆盖深度和广度的计算策略。
6. Ranking Fragment Ions Based on Outlier Detection for Improved Label-Free Quantification in Data-Independent Acquisition LC-MS/MS [O] . Aivett Bilbao, Ying Zhang, Emmanuel Varesio, -1

机译：基于离群值检测对碎片离子进行排序以改进独立于数据的采集LC-MS / MS中的无标记定量
7. Comparative Proteomic Analysis of Adhesion/Invasion Related Proteins in Cronobacter sakazakii Based on Data-Independent Acquisition Coupled With LC-MS/MS [O] . Ping Li, Xuan Dong, Xiao-yi Wang, 2020

机译：基于数据无关采集耦合LC-MS / MS的基于数据独立获取的Cronobacter Sakazakii中粘附/侵袭相关蛋白的比较蛋白质组学分析

Improved proteome coverage using novel data-independent acquisition strategies in ion mobility LC-MS experiments

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