Using neuroblastoma as an Alzheimer's disease model to study the effect of angiotensin converting enzyme inhibitors

摘要

Alzheimer's disease (AD) is the most common cause of dementia of elderly life. In order to understanding the relationship between AD and ACE inhibitors, the SH-SY5Y was treated with ACE inhibitors and the protein were analyzed by Reverse-phase-nano-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (RP-nano-HPLC-ESI-MS/MS) followed by peptide fragmentation patterning. In this study, three proteins, Low-density lipoprotein receptor-related protein 1B, Calreticulin and 14-3-3 protein zeta/delta, were identified and their expression were up-regulated. These proteins may contribute to the increase concentrations of beta-amyloid and phosphorylated Tau. Our results suggest that ACE inhibitors may play an important role in acceleration of the progression of clinical symptoms of AD.