Second-Generation Electron Transfer Dissociation (ETD) on the Thermo Scientific Orbitrap Fusion Mass Spectrometer with Improved Functionality, Increased Speed, and Improved Robustness of Data

摘要

A second-generation glow-discharge-based Easy-ETD source has been developed for the Orbitrap Fusion MS that incorporates significant hardware and software advancements. Calibration of the reaction kinetics removes the guesswork from ETD, and leads to conditions that optimizes the ETD reaction and scan cycle time. The calibrated reaction conditions are demonstrated to provide optimal conditions for ETD identifications and sequence coverage. Parallel acquisition provides ITMS~2 ETD scan rate cycle times of up to 12 Hz.