Investigating the bacterial proteome of M. tuberculosis using a combined discovery and targeted proteomics approach

摘要

1) Large-scale proteomics analyses successfully performed on complex bacterial extracts. 2) SCX fractionation of cell extracts aided in the removal of species that caused ion suppression. 3) Expression analyses of different protein classes showed distinct trends in regulation during hypoxia and re-aeration. 4) Combination of discovery and MRM workflows provided complementary data and extended proteome coverage of mtb bacterial proteins.