Ultra-fast Analysis of Benzodiazepines in Human Urine using Dilute and Shoot Methodology and SPE-MS/MS

摘要

Dilute and shoot feasibility was assessed using a panel of commercially available benzodiazepines. The MS response for the tested benzodiazepines was found to be linear over the 10 ng/mLto 5,000 ng/mL range with an R~(2) of at least 0.995 in each case. The limit of detection of the system was approximately 1 ng/mLfor the majority of compounds tested. The accuracies for each of the compounds were well within +-15percent, and usually less than 10percent, at all three QC concentrations (30, 2500, or 4000 ng/mL), suggesting that the system has a robust response over the entire range of concentrations tested. The precision on the QC samples was <10percent for the LQC and <5percent for the MQC and HQC samples, demonstrating the reproducibility of the analysis. These experiments establish the RapidFire-MS system as a viable and robust clinical research method for the detection of benzodiazepines in diluted human urine. The system further offers the advantage of vastly improved cycle time, as it achieves an analysis rate of approx9 seconds per sample (approx400 samples per hour) while producing data that is comparable to LC-MS/MS systems.