Mass spectrometric analysis of relative protein expression levels in glioblastoma multiforme tissues and lung cancer brain metastases

摘要

We identified 1,262 unique proteins from 5 sets of iTRAQ analysis after three iterative runs. 61 of which were significantly differently expressed; 23 proteins were overexpressed in GBM and 38 in the metastatic samples. A number of these proteins such as GFAP, vimentin and MARCKS are known to be differentially expressed in GBM, while others appear to be novel findings including PCM13 and PCM16, both of which were underexpressed, and PCM59 which was overexpressed in lung cancer brain metastasis. Western blot analysis of these biomarkers are currently undergoing. Future work will involve validation of these cancer biomarker using tissue microarray.