Mass Spectrometry Analysis of Ancient Bone from Columbian Mammoth

摘要

Demineralization, dialysis, and strong denaturants are not necessary to extract protein from fossilized bone. Less material and time can yield comparable results if utilizing a simple SDS extraction. Although HCD & ETD fragmentation methods yield less unique peptides than CID, HCD and ETD fragmentation provide critical information that is pivotal when de novo sequencing peptides.