'Mapping the HEPG2 Lipidome as effected by Niacin using Products of All Acquisition on an new generation Q Q TOF'

摘要

Recent developments in instrumentation and software have made it possible to acquire an array of data that can be mined for information of interest as opposed to designing an experiment to capture it directly. Data collected in an untargeted manner was mined for both expected and unexpected changes in the lipidome caused by niacin as reflected by the incorporation of a stable isotope label. An equivalent experiment using conventional acquisition methods was not possible. To demonstrate this new paradigm, HEPG2 cells were grown in the presence and absence of a stable labeled fatty acid and with and without the presence of niacin to observe the flux of the label into the various lipid pathways. Data were acquire on the Sciex TripleTOF 5600 using a scan function where precursor masses are iterated in 1 amu increments from m/z 200 to m/z 1000. Given the speed of the TOF it was possible to acquire ms/ms spectra for all 800 precursor ions in just under 3 minutes where each spectrum is the average of approximately 18 scans/precursor mass. All data were acquired using unit mass resolution for the precursor mass and approximately 40000 resolution of the product ion spectra, with collision energy ramping for each m/z event.