ATP Binding Cassette Transporter A1 Regulates the Lipid Raft Proteome of the Resting and Lipopolysaccharide-stimulated Macrophage

摘要

A semi-quantitative spectral counting approach was initially evaluated to study expression changes in lipid raft proteins of Abca1~(+/+) and Abca1~(-/-) bone marrow macrophages after LPS exposure. Two biological preparations showed significant variability. LC-MS/MS data indicate that several proteins are differentially up- or down-regulated in rafts from Abca1~(+/+) and Abca1~(-/-) macrophages. Further exploration of new biological replicates is underway to confidently define the global raft proteome and its dependence upon ABCA1 expression and LPS exposure. For better quantitation and confidence, a SILAC-based analysis of Abca1~(+/+) and Abca1~(-/-) bone marrow macrophages after LPS exposure is in progress.