New Reagents for Enhanced Charging of Intact Protein Ions for Liquid Chromatography Tandem Mass Spectrometry

摘要

Modifiers improved S/N ratio and enhanced charging of intact proteins for direct infusion and LC/MS experiments. The modifiers are compatible with LC and improved chromatographic resolution. The mechanism of charge enhancement may be correlated to the properties of the modifiers: e.g., higher boiling point may increase droplet lifetime and change protein conformation [4]. Further work is in progress to understand the mechanism of increased charge state and improved LC separation.