Robust and Sensitive Relative Quantitation of TMT Labeled E. coli Digest by Dual Cell Linear Ion Trap-Orbitrap Hybrid Mass

摘要

Optimal HCD settings for isobaric tag based quantitation using LTQ Orbitrap Velos are FT AGC MSn target at 50,000, isolation width of 1.2 amu, 1 uscan and 45percent NCE. Using optimal HCD settings more than 500 proteins were identified and quantified using HCD fragmentation in 40 ng of E. coli digest. In average 90percent of all identified peptides were quantified and about 83percent of these peptides with less than 15percent error.