Differentiation and sequencing of three constitutional isobaric 18-mer DNA oligomers using low-energy collision tandem mass spectrometry and the Mongo Oligo Mass Calculator

摘要

Mongo Oligo Mass Calculator Software facilitates the characterization and sequencing of DNA oligomers. Characteristic and distinct fingerprint product ion patterns permitted the discrimination amongst the individual 18-mer oligomers. Bidirectional sequencing using the product ions w, y, [a - B] and [d - H_(2)O]. Differentiation between the CID-MS/MS spectra of the 18-mer oligomers. Limitation: number of internal product ions makes the spectra more complexe to interpret.