Quantitation of Underivatized Acylcarnitines in Rat Liver Sample Extracts using Triple Quadrupole Mass Spectrometry

摘要

Many patients undergoing treatment for cancer will present conditions associated with reduced survival time and decreased response to therapy. One possible approach is a real-time monitoring of the therapeutic response to mitochondrial (beta)-oxidation. Mitochondrial (beta)-oxidation acts by decreasing carnitine-palmitoyl transferase I and II activities[1] which is reflected by fatty acid metabolism. Previous studies have linked mitochondrial fatty acid beta-oxidation disorders to specific enzyme or transporter deficiencies and acylcarnitine profiles have been used to facilitate the diagnosis of mitochondrial fatty acid (beta)-oxidation disorders in infants.[2] Here we present a method for the high sensitivity quantitation of underivatized acylcarnitines from rat liver extracts using positive mode tandem mass spectrometry without derivatization of the samples.