An Optimized UPLC-QTof-MS Method for Plant Metabolomics and Secondary Metabolism

机译：用于植物代谢组和次生新陈代谢的优化UPLC-QTOF-MS方法

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A new UPLC-QTof-MS method has been developed. This method was optimized for flow rate, column diameter, column length, column temperature and solvent gradient. Overall, the 2.1 X 150 mm column gave the best separation at a column temperature of 60 deg C and a flow rate of 560 (mu)L/min. The UPLC-QTof-MS method can be used for all plant tissue types - roots, leaf, stem and seed This method has applications across a broad range of secondary metabolites including saponins, glucosinolates and flavonoids.

机译：已开发出新的UPLC-QTOF-MS方法。该方法针对流速，柱直径，柱长，柱温和溶剂梯度进行了优化。总的来说，2.1×150mm列在塔温下的最佳分离，在60℃的柱温下，流速为560（mu）l / min。 UPLC-QTOF-MS方法可用于所有植物组织类型 - 根，叶，茎和种子该方法具有跨越广泛的次级代谢产物，包括皂苷，葡萄糖酸盐和黄酮类化合物。

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《American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics》|2009年||共1页
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David Huhman; Lloyd W. Sumner;
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中图分类 TB994.5-53;
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1. Metabolomics methods for the synthetic biology of secondary metabolism [J] . Quoc-Thai Nguyen, Merlo Maria E., Medema Marnix H., FEBS letters. . 2012,第15期

机译：二级代谢合成生物学的代谢组学方法
2. Metabolomics methods for the synthetic biology of secondary metabolism [J] . FEBS Letters . 2012,第15期

机译：二级代谢合成生物学的代谢组学方法
3. Development and Optimization of an UPLC-QTOF-MS/MS Method Based on an In-Source Collision Induced Dissociation Approach for Comprehensive Discrimination of Chlorogenic Acids Isomers from Momordica Plant Species [J] . Madala N. E., Tugizimana F., Steenkamp P. A. Journal of Analytical Methods in Chemistry . 2014,第Null期

机译：基于源碰撞诱导解离方法的UPLC-QTOF-MS / MS方法的开发和优化，用于全面鉴别苦瓜属植物绿原酸异构体
4. An Optimized UPLC-QTof-MS Method for Plant Metabolomics and Secondary Metabolism [C] . David Huhman, Lloyd W. Sumner American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2009

机译：用于植物代谢组和次生新陈代谢的优化UPLC-QTOF-MS方法
5. Investigation of plant specialized metabolism (secondary metabolism) using metabolomic and proteomic approaches. [D] . Xie, Zhengzhi. 2007

机译：使用代谢组学和蛋白质组学方法研究植物专门代谢（次级代谢）。
6. Development and Optimization of an UPLC-QTOF-MS/MS Method Based on an In-Source Collision Induced Dissociation Approach for Comprehensive Discrimination of Chlorogenic Acids Isomers from Momordica Plant Species [O] . N. E. Madala, F. Tugizimana, P. A. Steenkamp 2014

机译：基于源碰撞诱导解离方法的UPLC-QTOF-MS / MS方法的开发和优化用于全面鉴别苦瓜属植物绿原酸异构体
7. Development and Optimization of an UPLC-QTOF-MS/MS Method Based on an In-Source Collision Induced Dissociation Approach for Comprehensive Discrimination of Chlorogenic Acids Isomers fromMomordicaPlant Species [O] . N. E. Madala, F. Tugizimana, P. A. Steenkamp 2014

机译：基于源自碰撞诱导解离方法的UPLC-QTOF-MS / MS方法的开发和优化综合辨别型异常鉴别的综合辨别方法

An Optimized UPLC-QTof-MS Method for Plant Metabolomics and Secondary Metabolism

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