The Use of Recently Innovated Ambient Mass Spectrometry for Direct Determination of Pharmaceutical Compounds in Biological Matrices

摘要

LC-MS method has significant advantages in analytical speed, specificity and sensitivity over other approaches of chemical analysis. However the current approach is limited by the requirements of sample cleanup, chemical separation and inaccessible ionization in vacuum system, these has posed as major bottle necks in bioanalysis in drug discovery research. In this work, novel systems combining Atmospheric Pressure Solids Analysis Probe (ASAP) or Desorption electrospray ionization (DESI) with a triple-quadrapole mass spectrometer are developed and evaluated for direct bioanalysis of pharmaceuticals. The total analysis time could be potentially shortened from 4 minutes down to a few seconds per sample compared to conventional LC-MS/MS. The ultimate goal of the investigation is to provide optimized instrumentation designs and application procedures that are suitable for high throughput direct bioanalysis for pharmaceutical compound.