Microfluidic-based NanoLC/QQQ for high-sensitivity quantification of pharmaceutical molecules in small volumes of whole blood

摘要

The creation of new pharmaceutical drugs consumes vast amounts of capital. The competition is intense and cost savings become essential, even for leading pharma-companies. Substantial savings can be achieved by miniaturization of laboratory processes. A prime example of this situation exists in the DMPK-laboratory. Cost per PK-study may be decreased markedly by reduction in rodent size and number, which is accompanied by a cutback in the amount of active pharmaceutical ingredient per PK-study. Yet, small animals can only donate limited sample volumes especially when serial sampling studies are performed to increase PK-data quality. Here we present the high-sensitivity quantification of pharmaceuticals from 10 (mu)L of whole blood utilizing a microfluidic-based nanoflow LC system coupled to a triple quadrupole mass spectrometer.