Impact of flow-through regime on the cultivation of epithelial cells on quartz crystal resonators in micro fluidic channels

摘要

In this contribution the impact of culture medium flow on the cultivation of Madin-Darby canine kidney cells on quartz crystal resonators (QCRs) was studied. Cells were cultivated at different medium flow rates in a bioreactor chip containing 4 QCRs. Confluency and motility of cells were analyzed based on light microscopical pictures and impedance spectra of the resonators. Results were compared with cells cultivated in culture flasks mounted on a shaker and CFD simulation results of the bioreactor chip. Based on our studies the flow-regime in the micro fluidic channel geometry has major impact e.g. on the cell division rate.