Study of interaction in bioindication research on tobacco plant injuries caused by ground level ozone

摘要

Tropospheric ozone is one of the most phytotoxic air pollutants. Its concentration has increased over recent decades. Bioindication methods could help to measure ozone levels in places where automatic methods are not available. The investigations described were carried out in the city of Poznan (Poland) and in its vicinity, using tobacco plants as a bioindicator for ozone. Plants were located at 10 sites during the years 2002-2004, and visible ozone injuries were assessed. Statistical methods based on multidimensional models were used to interpret the results. The aim of this investigation was to evaluate the level of ozone-related injury to the 5th tobacco leaf, using multivariate analysis of variance. Differences of leaf injury levels between certain years and exposition sites were especially analyzed. The results of multivariate analysis were presented graphically in the space of canonical variates. The described study shows that multidimensional analysis of variance using canonical variates appears be an appropriate technique for testing hypotheses about differences between years and exposition sites as regards 5th leaf injury degree. The studies revealed a high variability of tobacco leaf injury caused by ozone in Poznan and its vicinity.