The Involvement of Cyclohexanone Monooxygenase Gene of Polaromonas sp. JS666 during Aerobic cis-Dichloroethene Degradation

摘要

Polaromonas sp. JS666 is the first aerobic bacterium capable of growth on cisdichloroethene (cDCE) as a sole carbon and energy source. It is being considered for use as a bioaugmentation agent at sites where cDCE has migrated into aerobic zones following the incomplete reductive dechlorination of higher-chlorinated ethenes in upgradient, anaerobic regions. In previous study, JS666 has shown two phenotypical behaviors of cDCEdegradation: one rapid and growth-coupled; and the other slower, cometabolic-like, exhibiting a transformation capacity. These observations, coupled with proteomic and transcriptomic analyses, suggest the existence of multiple cDCE-degradation pathways in JS666. One of the genes identified as upregulated during cDCE degradation encodes for a putative flavin-containing monooxygenase or cyclohexanone monooxygenase (cmo, bpro_5565). In JS666, it is likely that this protein catalyzes the formation of the cDCE-