Preparation of Salmon Atelocollagen Fibrillar Gel and Its Application to the Cell Culture

摘要

Salmon atelocollagen (SAC) has not been used as biomaterials due to its low denaturation temperature (19 degrees C). In the present study, we succeeded in preparation of SAC fibrillar gel stable at an actual physical temperature of human by cross-linking during fibril formation, and in cultivating human periodontal ligament cells on the cross-linked SAC fibrillar gel. Generally, collagens for biomaterials are prepared from mammalian sources, such as bovine and porcine skins. However, the use of mammalian sources has to be reconsidered and limited because of the risks of pathogens such as bovine spongiform encephalopathy (BSE). Although fish collagen is thought to be safe (1), it has not been widely used for biomaterials due to its low denaturation temperature.