QUANTITATION OF SERUM CREATININE IN MICE USING HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY (HILIC) COUPLED WITH HIGH-RESOLUTION ACCURATE-MASS (HRMS) ORBITRAP MASS SPECTROMETER

摘要

Serum creatinine level (SCR) is widely used as a biomarker of renal function. The SCRs are lower in normal mice than in human, dogs and rats. Colorimetric methods (Jaffe method and enzymatic method) commonly used in laboratory and clinic are insufficient from perspectives of accuracy and sensitivity for determination of the mouse SCR. A highly accurate and sensitive analytical method using LC/MS/MS for determination of mouse SCR has been developed.